human melanoma cell lines skmel28  (ATCC)

Journal: Genes & Development

Article Title: Fatty acid uptake activates an AXL–CAV1–β-catenin axis to drive melanoma progression

doi: 10.1101/gad.351985.124

Figure Lengend Snippet: Reagents used in this study

Article Snippet: SKmel28 (male) human melanoma cell line , ATCC , , CVCL_0526.

Techniques: Software, Transduction, Virus, Subcloning, Bacteria, Recombinant, Bradford Assay, Protease Inhibitor, RNA Extraction, Reporter Assay, Bicinchoninic Acid Protein Assay, Quantitative RT-PCR, Negative Control

Journal: STAR Protocols

Article Title: Protocol for high-precision CRISPR-Cas12a-based SNV detection on synthetic DNA, cell line cfDNA models, and liquid biopsies

doi: 10.1016/j.xpro.2025.103696

Figure Lengend Snippet:

Article Snippet: Human cell line SKmel28 , ATCC , Cat#HTB-72; RRID: CVCL_0526.

Techniques: Recombinant, Modification, Purification, Variant Assay, Amplification, Software

Journal: Cell communication and signaling : CCS

Article Title: Revealing the role of RAB27 in HER receptor family expression and signaling in melanoma cells.

doi: 10.1186/s12964-025-02064-8

Figure Lengend Snippet: Fig. 1 Expression of cancer-related proteins in wild-type (WT) and RAB27A knockout (KO) SkMel28, DMBC12 and A375 cells. Signal intensity was quanti fied by densitometric analysis and data are presented as mean values of two technical replicates. Proteins whose signal exceeded the OD value of 3000 and the difference between WT and KO was at least 20% were grouped according to their function

Article Snippet: The human melanoma SkMel28 cell line was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Knock-Out

Journal: Cell communication and signaling : CCS

Article Title: Revealing the role of RAB27 in HER receptor family expression and signaling in melanoma cells.

doi: 10.1186/s12964-025-02064-8

Figure Lengend Snippet: Fig. 3 Expression of HER receptors in wild-type (WT) and RAB27A knockout (KO) SkMel28, DMBC12 and A375 cells and RAB27A/B knockout (dKO) A375 cells measured using the Proteome Profiler array. Signal intensity was quantified by densitometric analysis and data are presented as mean OD values of two technical replicates

Article Snippet: The human melanoma SkMel28 cell line was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Knock-Out

Journal: Cell communication and signaling : CCS

Article Title: Revealing the role of RAB27 in HER receptor family expression and signaling in melanoma cells.

doi: 10.1186/s12964-025-02064-8

Figure Lengend Snippet: Fig. 4 RAB27A knockout altered the levels of EGFR, HER2 and HER3 in wild-type (WT) and RAB27A knockout (KO) SkMel28, DMBC12 and A375 cells. (A) mRNA expression analyzed by RT-PCR. Data presented as mean ± SD, n ≥ 3, p < 0.05 (B) Receptor occupancy analyzed by flow cytometry. Data presented as mean ± SD, n ≥ 4, p < 0.05 (C) Representative flow cytometry (D) Protein expression analysed by Western blot. The acquired bands were quantified by densitometric analysis. Data presented as mean ± SD, n ≥ 5, p < 0.05 (E) Representative Western blot

Article Snippet: The human melanoma SkMel28 cell line was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Knock-Out, Expressing, Reverse Transcription Polymerase Chain Reaction, Flow Cytometry, Western Blot